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81.
82.
The biotransformation of pregnenolone-7alpha-3H and of progesterone-7alpha-3H by porcine fetal and maternal adrenal homogenates at 56 and 112 days of pregnancy and of dehydroepiandrosterone-7alpha-3H by fetal adrenal homogenates has been investigated in vitro. Both pregnenolone-7alpha-3H and progesterone-7alpha-3H were metabolized extensively by maternal adrenal preparations, the principal radioactive metabolites isolated being cortisol, corticosterone, 11-deoxycortisol, deoxycorticosterone, 11beta-hydroxyprogesterone and androstenedione. In addition, 17alpha-hydroxyprogesterone, 20alpha-dihydroprogesterone and cortisone were formed from both substrates and 17alpha-hydroxypregnenolone and progesterone were formed from pregnenolone. Although essentially the same radioactive metabolites were isolated after incubation of fetal adrenal glands with pregnenolone-7alpha-3H or progesterone-7alpha-3H, a greater proportion of the radioactivity was associated with corticosteroids at 112 days of pregnancy than at 56 days. 11beta-Hydroxyandrostenedione and androstenedione were isolated and identified together with an unknown polar metabolite, after incubation of fetal adrenal tissue with dehydroepiandrosterone-7alpha-3H. These results are discussed in relation to feto-placental steroid biosynthesis and metabolism and the role of the fetal adrenal in the initiation of parturition in the pig. 相似文献
83.
Ravi babu. Kalahasthi Rajmohan. Hirehal Raghavendra rao Rajan. Bagalur Krishna murthy 《Biomarkers》2013,18(3):241-249
The objectives were to investigate the plasma lipid peroxidation and erythrocyte antioxidants status in workers exposed to nickel. The study groups comprised 69 nickel plating workers and 50 office workers residing in the same city, but away from the place of work of the study group subjects, considered as control group. Urinary nickel concentration was determined by graphite furnace atomic absorption spectrophotometry. The plasma lipid peroxidation and erythrocyte antioxidants were measured by spectrophotmetric methods. The plasma lipid peroxidation level was significantly increased in nickel-platers and their helpers as compared with controls. Erythrocyte antioxidants were significantly decreased in the nickel-platers compared with the controls. The level of plasma lipid peroxidation was positively and erythrocyte antioxidants were negatively and significantly correlated with the urine nickel levels. Multiple regression analysis assessed the oxidative stress associated with nickel and other potential confounding factors such as body mass index, the consumption of green vegetables, coffee, tea, smoking and alcohol consumption. Analysis showed that the lifestyle confounding factors: the consumption of green vegetables, smoking and alcohol, were not significantly associated with oxidative stress. The exposure to nickel, body mass index and coffee consumption were significantly associated with oxidative stress. The results show that the increased plasma lipid peroxidation and decreased erythrocyte antioxidants levels observed in nickel-exposed workers could be used as biomarkers of oxidative stress. 相似文献
84.
Nickel is harmful to humans, being both carcinogenic and allergenic. However, the mechanisms of this toxicity are still unresolved. We propose that Ni(II) ions disintegrate proteins by hydrolysis of peptide bonds preceding the Ser/Thr‐Xaa‐His sequences. Such sequences occur in nuclear localization signals (NLSs) of human phospholipid scramblase 1, Sam68‐like mammalian protein 2, and CLK3 kinase. We performed spectroscopic experiments showing that model nonapeptides derived from these NLSs bind Ni(II) at physiological pH. We also proved that these sequences are prone to Ni(II) hydrolysis. Thus, the aforementioned NLSs may be targets for nickel toxicity. This implies that Ni(II) ions disrupt the transport of some proteins from cytoplasm to cell nucleus. 相似文献
85.
Zhuo Yu Bingliang Wang Xiaobin Liao Kangning Zhao Zhifang Yang Fanjie Xia Congli Sun Zhuo Wang Chaoying Fan Jingping Zhang Yonggang Wang 《Liver Transplantation》2020,10(25)
Lithium–sulfur batteries have attracted extensive attention because of their high energy density. However, their application is still impeded by the inherent sluggish kinetics and solubility of intermediate products (i.e., polysulfides) of the sulfur cathode. Herein, graphene‐supported Ni nanoparticles with a carbon coating are fabricated by directly carbonizing a metal–organic framework/graphene oxide composite, which is then dispersed on a commercial glass fiber membrane to form a separator with electrocatalytic activity. In situ analysis and electrochemical investigation demonstrate that this modified separator can effectively suppress the shuttle effect and regulate the catalytic conversion of intercepted polysulfides, which is also confirmed by density functional theory calculations. It is found that Ni–C sites can chemically interact with polysulfides and stabilize the radical S3?? through Ni? S bonds to enable fast dynamic equilibrium with S62?, while Ni nanoparticles reduce the oxidation barrier of Li2S and accelerate ion/electron transport. As a result, the corresponding lithium–sulfur battery shows a high cycle stability (88% capacity retention over 100 cycles) even with a high sulfur mass loading of 8 mg cm?2 and lean electrolyte (6.25 µ L mg?1). Surprisingly, benefitting from the improved kinetics, the battery can work well at ?50 °C, which is rarely achieved by conventional Li–S batteries. 相似文献
86.
One of the most promising applications of nanomaterials is that of nanobiosensors, using biomolecules such as nucleic acids as receptors. This study aimed to synthesize nickel oxide nanoparticles (NiO NPs) by an environmentally friendly green synthesis, using the extract of the herb Coriandrum sativum (coriander). The synthesized NPs were characterized using UV–Visible spectroscopy, Fourier transform infrared spectroscopy, X‐ray diffraction, X‐ray photon spectroscopy, field emission scanning electron microscopy coupled with energy dispersive spectroscopy, dynamic light scattering, zeta potential and transmission electron microscopy. All results confirmed the synthesis of pure, spherical, positively charged NiO NPs of around 95 nm in diameter with prominent hydroxyl groups attached to the surface. Furthermore, interaction studies of synthesized NiO NPs with calf thymus DNA (CT DNA) were performed using UV–Visible spectroscopy, UV–thermal melting, circular dichroism, and fluorescence spectroscopy. CT DNA served as a substitute for nucleic acid biosensors. All experimental studies indicated that the NiO NPs bound electrostatically with CT DNA. These studies may facilitate exploring the potential of NiO NP–nucleic acid conjugated materials to be used as nanobiosensors for various applications, especially in pharmacological, epidemiological, and environmental diagnostic applications, and in detection. 相似文献
87.
Environmental pollution is currently identified as one of the major drivers of rapid decline of insect populations, and this finding has revitalized interest in insect responses to pollution. We tested the hypothesis that the pollution-induced decline of insect populations can be predicted from phenotypic stress responses expressed as morphological differences between populations inhabiting polluted and unpolluted sites. We explored populations of the brassy tortrix Eulia ministrana in subarctic forests along an environmental disturbance gradient created by long-lasting severe impacts of aerial emissions of the copper–nickel smelter in Monchegorsk, northwestern Russia. We used pheromone traps to measure the population densities of this leafrolling moth and to collect specimens for assessment of three morphological stress indices: size, forewing melanization, and fluctuating asymmetry in wing venation. Wing length of E. ministrana increased by 10%, and neither forewing melanization nor fluctuating asymmetry changed from the unpolluted forest to the heavily polluted industrial barren. However, the population density of E. ministrana decreased 5 to 10 fold in the same pollution gradient. Thus, none of the studied potential morphological stress indicators signaled vulnerability of E. ministrana to environmental pollution and/or to pollution-induced environmental disturbance. We conclude that insect populations can decline without any visible signs of stress. The use of morphological proxies of insect fitness to predict the consequences of human impact on insect populations is therefore risky until causal relationships between these proxies and insect abundance are deciphered. 相似文献
88.
Laura Pancaro Mario Innamorati Ornella Vergnano Gambi Silvia Occhiochiuso 《Plant biosystems》2013,147(6):265-284
Abstract Cobalt, nickel and chromium effects on germination of Alyssum, during after-ripening and aging. Cobalt (2.5–20mM), nickel (2.5–25mM) and chromium (0.2-1mM) effects have been studied in seeds of three species of Alyssum, of which two, A. bertolonii and A. argenteum, endemics to serpentine soils and one, A. nebrodense, closely related to the others but endemic to the Madonie (Sicily) where it grows on limestone. The results, expressed as percentage of germinated seeds (p), Kotowski coefficient of velocity (v) and germination average ratio (pτ), have been subjected to statistical analysis. The effect per mM is generally inhibitory, not significant for cobalt, weak but significant for nickel, more marked and significant for chromium, linearly correlated to concentration. The inhibition reaches its highest level when seeds are ripe, being significantly related to the control germination capacity (pτ). The values of the specific inhibition (effect/mM modditppτ) for all the three species are higher for chromium, which is more toxic than nickel, and increase from A. nebrodense to A. argenteum, showing that of the two serpentine endemics A. bertolonii is more tolerant both to nickel and chromium. 相似文献
89.
Hao Chen Linfeng Hu Yan Yan Renchao Che Min Chen Limin Wu 《Liver Transplantation》2013,3(12):1636-1646
A facile one‐step hydrothermal co‐deposition method for growth of ultrathin Ni(OH)2‐MnO2 hybrid nanosheet arrays on three dimensional (3D) macroporous nickel foam is presented. Due to the highly hydrophilic and ultrathin nature of hybrid nanosheets, as well as the synergetic effects of Ni(OH)2 and MnO2, the as‐fabricated Ni(OH)2‐MnO2 hybrid electrode exhibits an ultrahigh specific capacitance of 2628 F g?1. Moreover, the asymmetric supercapacitor with the as‐obtained Ni(OH)2‐MnO2 hybrid film as the positive electrode and the reduced graphene oxide as the negative electrode has a high energy density (186 Wh kg?1 at 778 W kg?1), based on the total mass of active materials. 相似文献
90.
Escherichia coli topoisomerase I (EcTopoI) is a type IA bacterial topoisomerase which is receiving large attention due to its potential application as novel target for antibacterial therapeutics. Nevertheless, a detailed knowledge of its mechanism of action at molecular level is to some extent lacking. This is partly due to the requirement of several factors (metal ions, nucleic acid) to the proper progress of the enzyme catalytic cycle. Additionally, each of them can differently affect the protein structure. 相似文献